Liver Allograft Biopsy - Case 29

<HTML>  <HEAD><title>Consult Report - Case 29</title></HEAD> <body bgcolor=#ffffff link="darkblue" vlink="006666"> <IMG SRC="/tpis/icons/purpball.gif"><i> Contributed by Anthony Demetris, M.D.</A></i> <HR> <b>PATIENT HISTORY:</b> Per referral letter, the patient is a 57-year-old Saudi male with end stage liver disease due to Hepatitis B who was transplanted on 12/2/96. He has had good liver functions until quite recently. He developed a persistent fever which has defied every work up. He has been cultured from every site, scanned from head to toe, surgically explored, and biopsied in multiple locations.<P> An excisional biopsy of a supraclavicular lymph node turned out to be a localized area of necrosis within the subcutaneous tissue. There is no real granuloma formation or evidence of a pre-existing lymph node. All cultures have been negative. No evidence of PTLD. He has been imperically treated with numerous antibiotics, anti-fungal agents, and anti-tuberculous agents--all without result. Currently, his serum chemistries are TB 15.9; AP 255; GGT 345; AST 340; ALT 276, ALB 2.9; PT 13.8. His immunosuppression has been discontinued. Review of outside material. <hr> <a name="final"></a> <H4>Final Diagnosis (Case 29) [D808-97]</H4> LIVER ALLOGRAFT, NEEDLE BIOPSY -<BR> <ol type=A> <li>MILD ACUTE CELLULAR REJECTION WITH PROMINENT CENTRAL VENULITIS AND FOCAL HEPATOCELLULAR DROPOUT. <li>LOBULAR DISARRAY, KUPFFER CELL HYPERTROPHY AND HEPATOCYTE REACTIVE CHANGES; HEPATITIS B VIRUS INFECTION AND SEPSIS SHOULD BE EXCLUDED (SEE MICROSCOPIC DESCRIPTION). <li>PORTAL AND PERIPORTAL HEMORRHAGE, CAUSE UNCERTAIN. </ol><p> <i><b>Previous Biopsies on this Patient:</b></i><br> NONE<p> <i><b>TPIS Related Resources:</b></i><br> <a href="/tpislibrary/schema/BanffRej.html">Liver Allograft Rejection Grading</a><br> <a href="/tpislibrary/liver/index.html">Liver Transplant Topics</a><p> <a name="gross"></a> <hr><H4>Gross Description - Case 29 [D808-97]</H4> The specimen consists of three (3) consult slides labeled D808-97 (2) and D50-97 (1). No surgical pathology report is received with the specimen. <p> <a name="micro"></a> <HR><H4>Microscopic Description - Case 29 [D808-97]</H4> <A HREF="/tpis/images/H00056a.jpg"><IMG SRC="/tpis/images/tnails/H00056a.jpg" width=133 height=100></a> <A HREF="/tpis/images/H00056b.jpg"><IMG SRC="/tpis/images/tnails/H00056b.jpg" width=133 height=100></a> <A HREF="/tpis/images/H00056c.jpg"><IMG SRC="/tpis/images/tnails/H00056c.jpg" width=133 height=100></a> <A HREF="/tpis/images/H00056d.jpg"><IMG SRC="/tpis/images/tnails/H00056d.jpg" width=133 height=100></a> <P> The normal lobular architecture is distorted by portal expansion because of edema and focal hemorrhage. There is also a mild focal, predominantly mononuclear portal infiltrate and focal bile duct infiltration and damage are identified.<P> The most striking changes are in the lobule. There is zonal centrilobular hepatocellular dropout, lymphoplasmacytic inflammation, congestion and hemorrhage. Focal central venulitis is also appreciated. Mild perivenular fibrosis is seen. However, there is also marked Kupffer cell hypertrophy, and reactive changes of hepatocytes including an increased nuclear:cytoplasmic ratio, spotty acidophilic necrosis of hepatocytes, and macronucleoli.<P> Compared to the previous biopsy there has been a marked increase in the centrilobular dropout and inflammation along with the congestion and hemorrhage.<P> Overall, the histopathological changes suggest that more than one process is contributing to the pathologic changes. First, the zonal centrilobular dropout with central plasmacytic inflammation and focal lymphocytic duct damage suggests a component of acute cellular rejection with central venulitis. However, it is difficult to explain the panlobular disarray, marked hepatocyte reactive changes, and Kupffer cell hypertrophy on the basis of rejection alone. These changes can be seen with hepatitis B viral infection of the liver, sepsis and reactive/regenerative change from vascular compromise, which last of which appears to have been excluded by imaging studies. Immunohistochemical stains to exclude HBV infection are suggested.<P> Lastly, the cause of the portal and periportal hemorrhage is uncertain. I have seen this change in intra-operative needle biopsies after vigorous manipulation of the liver for operative access, which presumably causes torsion between the portal tracts and the surrounding, less rigid, parenchyma. Other causes of portal and periportal hemorrhage include DIC and humoral rejection. However, none of these possibilities appear to be viable options based on the clinical profile and other histopathological findings. <P>  <hr> <font size=3> Please mail comments, corrections or suggestions to the <a href="mailto:harperth@upmc.edu"> <i>TPIS administration</i></a> at the UPMC.<p> This page and its contents are Copyright © 1996, 1997 <A HREF="http://www.upmc.com/Pages/default.aspx">University of Pittsburgh</a>. All rights reserved. Unauthorized redistribution prohibited.<p> </body> </html>